In vivo reconstitution of neutrophils at the tissue site restores experimental BP in Mac-1-deficient mice. Mac-1 KO mice were injected intradermally with either pathogenic anti-mBP180 IgG R530 alone (bar 1), pathogenic IgG plus 5 × 105 WT (bar 2), or Mac-1 KO neutrophils (bar 3). The animals were examined 24 hours after IgG injection. The Mac-1 KO mice reconstituted with neutrophils from WT (bar 2) or Mac-1 KO mice (bar 3) developed blisters and the lesional skin of the diseased mice had a significantly higher number of neutrophils than the nonlesional skin of the Mac-1 KO mice without neutrophil reconstitution (bar 1). Data shown are the mean ± SEM. n = 6 for each group. *P < .05 (bar 1 vs bar 2; bar 2 vs bar 3); **P < .001 (bar 1 vs bar 3).
