T lymphocytes redirected to express the κ-specific CAR eliminate Igκ⁺dimtumor cells. T lymphocytes obtained from healthy donors and transduced either with control-GFP vector (A), CAR46ζ (B), or CAR46/28ζ (C) were cocultured with the CCL-120 tumor cell line (ratio, 5:1), which is Igκ^+dim. After 5 to 7 days of culture, cells were collected and stained with CD3-PerCP and CD19-PE to evaluate the growth of CD19⁺ tumor cells. No CD19⁺ cells were detectable after coculture with CAR46ζ⁺ or CAR46/28ζ⁺ T lymphocytes, whereas CD19⁺ cells were detectable when tumor cells were cocultured with control T cells.