Figure 3.
Detection of multiplied SRCs in SFSD culture. (A) Experimental protocol for the detection of multiplied SRCs in SFSD culture. (B) PCR tracing of individual clones in mice that underwent transplantation with SFSD-cultured cells. Representative clones are shown. Each clone was detected by amplifying a unique genomic-proviral junction sequence with primer pairs located in the genome (clone number shown in Table S1) and vector (LTR3). For semiquantitative PCR, genomic DNA from the indicated ratio of mixture of Jurkat cells with and without transduction by recombinant retrovirus MFG-EGFP22 was used. The numbers at the top indicate each mouse. The numbers on the left indicate each SRC clone. P indicates positive control; N, negative control.
