Detection of self-renewal and multi-lineage differentiation by multiplied SRCs. (A) Experimental protocol for the detection of self-renewal and multilineage differentiation by multiplied SRCs. (B) PCR tracings of individual clones in bone marrow cells from mice that underwent transplantation with SFSD-cultured cells are shown in column 1. The bone marrow cells were then transplanted into secondary mice. PCR tracings of individual clones in CD19⁺EGFP⁺ and CD33⁺EGFP⁺ cells from the secondary mice are shown in column 2. Each clone was detected by amplifying a unique genomic-proviral junction sequence with primer pairs located in the genome (clone number shown in Table S1) and vector (LTR3). The numbers at the top indicate each mouse. The numbers on the left indicate each SRC clone.