F36VMpl accelerates hematologic recovery following myeloablative irradiation. Twelve mice exposed to myeloablative irradiation (1050 cGy) were given transplants of either 7.6 × 105 F36VMpl-transduced (circles) or 6.6 × 105 F36VFGFR1-transduced (triangles) marrow cells, with gene transfer rates into progenitors of 65.6% and 46.6%, respectively. Beginning immediately following the infusion of transduced marrow cells, half of the mice in each group were treated with daily injections of AP20187 (10 mg/kg) for 3 doses (+). – indicates no CID. Complete blood counts were obtained 12 days after transplantation. (A) Complete blood counts. White cells were spuriously elevated in CID-treated mice that were given transplants of F36VMpl-transduced marrow cells due to the incorrect assignment of reticulocytes by the particle counter. ○ indicates measurements obtained from a normal control mouse. (B) Percentages of GFP+ red cells, platelets, WBCs, polymorphonuclear cells (polys), monocytes (monos), and lymphocytes (lymphs). Values reflect means of 3 mice. Error bars indicate SDs. All P values reflect differences between CID-versus non-CID–treated mice that were given transplants of F36VMpl-transduced marrow cells.
