^Gγ-globin is preferentially activated by TSA and anisomycin. (A) Quantitative data obtained by qPCR analysis with ^Gγ-globin-specific primers are shown in the graph (see “Materials and methods”). The fold increase in ^Gγ-globin/GAPD mRNA ratio (□) is shown for NaB, TSA, and anisomycin treatments in the absence or presence of SB203580 (Sb) pretreatment (▪). Control studies with 0.4% dimethyl sulfoxide (DMSO) were completed. The levels in untreated (UT) K562 cells were normalized to 1. Data are shown as the mean ± SEM. The star above the graph (comparison between UT and treated cells) or the bracket and star (comparison between the different treatment conditions indicated) represent significance at the P < .05 level. (B) Analogous studies were performed to determine ^Aγ-globin/GAPD mRNA ratios (□) under the same experimental conditions.