The G-CRE is required for ^Gγ-promoter activation by HDACIs. (A) Shown is a schematic diagram of the reporter plasmids established with ^Gγ-promoters truncated at nucleotides -1500, -1350, and -1180. (B) K562 cells were transfected with reporters along with β-galactosidase to control for transfection efficiency. Transfected K562 cells were treated with HDACIs in the absence (-) or presence (+) of 10 μM SB203580. The relative luciferase activity was calculated after correcting for total protein and β-galactosidase activity. The untreated samples (-, □) were normalized to 1. Data for NaB or TSA in the absence (▪) or presence () of SB203580 are shown as the mean ± SEM. The star and brackets are the same comparisons as defined in Figure 4A.