NUP98-HOX/Meis1 and NUP98-HOX/Flt3 bm cells show similar signal transduction properties. The indicated bm cells were starved for 16 hours in 0.5% FBS medium and then stimulated with starve media (labeled 0) or with nonsaturating concentrations (labeled *) of hIL-6, mIL-3, mSCF, and FL (A) or with FL alone (B) for 5 minutes at 37°C. Total-cell lysates were separated by SDS-PAGE, transferred to PVDF, and probed with antibodies for p-Akt ser 473, p-Erk1/2, or GAPDH as a loading control.