American Society of Hematology

Figure 1.

$Figure 1. Arfs are present in platelets. (A) Recombinant HA-Arf1 and HA-Arf6 proteins were immunoblotted with anti-Arf1/3 and anti-Arf6 antibodies. (B) Platelet extract (50 μg) and HEK 293 cell extract (10 μg) were immunoblotted with anti-panArf, anti-Arf1/3, and anti-Arf6 antibodies. (C) Washed platelets (4 × 108/mL) were sonicated and fractionated by centrifugation. The pellet and the supernatant fractions were immunoblotted with anti-RabGDI, anti–syntaxin 4, anti-Arf6, and anti-Arf1/3 antibodies.$

Arfs are present in platelets. (A) Recombinant HA-Arf1 and HA-Arf6 proteins were immunoblotted with anti-Arf1/3 and anti-Arf6 antibodies. (B) Platelet extract (50 μg) and HEK 293 cell extract (10 μg) were immunoblotted with anti-panArf, anti-Arf1/3, and anti-Arf6 antibodies. (C) Washed platelets (4 × 10⁸/mL) were sonicated and fractionated by centrifugation. The pellet and the supernatant fractions were immunoblotted with anti-RabGDI, anti–syntaxin 4, anti-Arf6, and anti-Arf1/3 antibodies.

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