Myr-Arf6 peptide affects platelet aggregation. Washed platelets were incubated with DMSO (vehicle) or Arf peptides for 2 minutes with stirring at 37°C, followed by stimulation with 10 μg/mL collagen (B-C) or 0.1 μg/mL convulxin (D-E). (A) Sequences of the Arf peptides used. (B,D) Different doses of myr-Arf6 (gray line) were preincubated for 2 minutes prior to stimulation, and the aggregation traces were aligned with those from reactions containing an equal amount (1%) of DMSO (black line). (C,E) Myr-Arf1 (25 μM), nonmyr-Arf6 (25 μM), and DMSO were preincubated for 2 minutes prior to the stimulation.