Figure 5.
Myr-Arf6 peptide affects Arf6-GTP hydrolysis. (A) Washed platelets (4 × 108/mL) were incubated with DMSO (–) or myr-Arf6 peptide (+, 25 μM) for 2 minutes with stirring at 37°C, followed by stimulation with 10 μg/mL collagen. After incubation for the indicated times, platelets were lysed and the supernatants were analyzed for Arf6-GTP. (B) Washed platelets were incubated with DMSO or indicated Arf peptides (25 μM) for 2 minutes with stirring at 37°C, followed by stimulation with 0.1 μg/mL convulxin. After incubation for the indicated times, platelets were lysed and the supernatants were analyzed for Arf6-GTP.
