Western blot analysis of VWF multimers in plasma after tail vein administration of the murine VWF cDNA to VWF^–/– mice. Murine VWF cDNA (100 μg) was administered to VWF^–/– mice with saline as a negative control. After 48 hours, plasma was collected from mice that had received injections and was assessed by Western blot analysis. Relative gel positions of VWF multimer bands are indicated on the right. Lane 1, plasma of naive wild-type mice showing normal VWF multimer pattern; lane 2, plasma from naive VWF^–/– mice showing the absence of VWF multimers; lane 3, plasma from VWF^–/– mice 48 hours after administration of saline showing the monomer/dimer pattern; lane 4, VWF^–/– mice 48 hours after administration of 100 μg eGFP cDNA showing the absence of VWF multimers; lane 5, plasma from VWF^–/– mice 48 hours after administration of 250 μg VWF cDNA; lane 6, plasma from VWF^–/– mice 48 hours after administration of 100 μg VWF cDNA; lane 7, plasma from VWF^–/– mice 48 hours after administration of 50 μg VWF cDNA. Lanes 5 to 7 reveal a VWF pattern similar to that seen in WT mice.