Relative expression of the genes in the 6q23 candidate interval throughout phase II. Gene transcription was determined by real time RT-PCR. Gene expression levels are related to the gene expression of one sample after HPRT normalization. (A) Relative expression of ALDH8A, AHI1, PDE7B, HBS1L, cMYB, and cMYB-9A. Each data point represents the mean value for each individual in the HPFH and control groups. For day 7, values were obtained for only 11 of the 12 HPFH, and 13 of the 14 controls. There were inadequate cell numbers for analysis for culture of one individual in each group. (B) Correlation of cMYB with cMYB-9A or HBS1L expression. The Ct values are a measure of the amount of template mRNA, the lower the Ct value, the higher the amount of mRNA template. The data points include all values, with more than one value for 6 individuals who had repeat erythroid cultures. (C) Western blot analysis of total cell lysates from phase II erythroid cell cultures. Samples 1 and 2 represent 2 different individuals to illustrate the pattern of the cMYB protein expression in phase II. Pos refers to the cell lysate from K562 cells.