Figure 3.
![Figure 3. Genomic PCR-SSCP analysis of RFC exon 3 in parental cells and their PT523-resistant sublines. High-molecular-weight gDNA (∼50 ng) isolated from parental CCRF-CEM cells and their various antifolate-resistant sublines was PCR amplified in the presence of [32P]dCTP using oligonucleotide primers targeting exon 3. The PCR products were resolved by electrophoresis on nondenaturing, 8% polyacrylamide gels, as described in “Materials and methods.” The arrowhead shown on the right side depicts the band with the altered electrophoretic mobility.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/107/8/10.1182_blood-2005-10-4048/4/zh80080694130003.jpeg?Expires=1736039616&Signature=c8aw7q41mk0eMAFm7qhOww0BgwGScoHQ-NxE1sSRcd3HoOcKo1bJCqRTPvjHzgDbuGarpepa7juuadefU3ZE-QS1leNpJfI4FSreaoWbeZk~gMAAcvcQL-HU3nrNaOyjvCtUhKGMsUI6hzaJC5k~qGiXGbnRWqijou3ReRupR2ONveWJeDH1UWaN1-LcvzdSrnTJAspNvFPJ5cTvMX959x6Y6Phx46Bp-j9I~h456ImXi8sxuxU3WbqVBWgI7I8Ex3w1fTCfPbkVfsKoh6wtwQzahBhWb65mggU4HrvhoYcF12TcUmzi3GENCA44lNSM4hfg1U~7jXlY7hfg1hX5yA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Genomic PCR-SSCP analysis of RFC exon 3 in parental cells and their PT523-resistant sublines. High-molecular-weight gDNA (∼50 ng) isolated from parental CCRF-CEM cells and their various antifolate-resistant sublines was PCR amplified in the presence of [32P]dCTP using oligonucleotide primers targeting exon 3. The PCR products were resolved by electrophoresis on nondenaturing, 8% polyacrylamide gels, as described in “Materials and methods.” The arrowhead shown on the right side depicts the band with the altered electrophoretic mobility.
