Effect of stimulation of hMCs with PGE2 and receptor-selective analogs on cAMP accumulation. (A) Effect of PGE2. Samples of 2 × 105 hMCs were stimulated for 10 minutes with the indicated concentrations of PGE2. Values are mean ± SEM of triplicate samples from a single experiment. Similar results were obtained with hMCs from 2 additional donors. (B) Effect of selective EP receptor agonists. Cells were stimulated for 10 minutes with the indicated concentrations of selective agonists of the EP1 (D1-004), EP2 (AE1-259-01), EP3 (AE-248), and EP4 (AE-329) receptors, or with DMSO alone. Values are expressed as absolute quantities of cAMP and are expressed as mean ± SEM for 5 experiments. (C) Effect of the EP4 receptor-selective antagonist AE-208 (10 μM) on cAMP accumulation in response to hMC stimulation with PGE2 (1 μM) or AE-329 (10 μM). Values are expressed as percent of control (DMSO alone) and are mean ± SEM from 4 experiments. *Significant.
