Impact of αvβ5 ligation on DC maturation. DCs were incubated with αvβ5- or Ig-ApoS, after which noninternalized Apo-S was lysed and MCM-mimic, LPS, or LPS and IFNγ were added to the cultures for 40 hours. (A) Expression of MHC I, MHC II, CD80, CD86, and CD83 molecules on DCs. Mean fluorescence intensity and increase over immature DCs (gray histograms) are shown for each sample. (B) Following preexposure to ApoS, DCs were exposed to LPS for 40 hours or left immature and then cocultured for 1.5 hours with increasing concentrations of FITC-dextran beads. Uptake of beads by αvβ5-ApoS–exposed versus control DCs was measured by FACS. Percentage of dextran-FITC–positive, MHC I (PE)–gated DCs is shown for immature (•, ▴) versus LPS-matured DCs (○, ▵). (C) Culture supernatants were collected and analyzed for the presence of IL12, TNFα, IL1β, and IL6. Error bars represent SD of duplicate wells in ELISA plate. Representative experiments of at least 3 performed are shown.