Regulators of transcription and survival are affected by RAX expression during IFNγ/TNFα treatment. (A) Western blotting was used to monitor IκBα degradation and IRF-1 production after cells had been treated with 10 ng/mL IFNγ followed by treatment with 10 ng/mL TNFα for the indicated times. (B) The level of STAT1 and phosphoserine 727 STAT1 were monitored after IFNγ/TNFα treatment by Western blotting.