TLR8 agonists induce robust IL-12 p40/70 production from adherent monocytes. PBMCs were allowed to adhere onto plastic wells (1.5 × 10⁶ PBMCs/well) and cultured in 10% fresh autologous serum prior to addition of TLR agonists and incubation at 37°C/5% CO₂ for 48 hours. IL-12 p40/70 production was measured in culture supernatants by ELISA. Data represent means ± SEM. Statistical comparisons were made within each group in relation to 500 μM of the TLR7 agonist imiquimod (open arrow, newborns; filled arrow, adults). n = 4; *P < .05.