Figure 6.
Reduced levels of RAX or expression of RAX(S18A) promotes anchorage-independent colony formation. MEF cells with reduced RAX or expressing exogenous RAX or RAX(S18A) were assayed for colony formation after 14 days of growth in soft agar. A representative crystal violet–stained plate for each cell line is shown. The transformation efficiency for each cell line was determined by averaging the number of colonies formed (groups of approximately 50 or more cells) from 4 separate plates divided by the total number of cells plated (1 × 104) ± SD.
