Intrathymic T-cell development in γδ^– × hδTg mice. (A) Thymocytes and splenocytes from WT and γδ^– × hδTg mice were surface stained with anti-CD4 and anti-CD8 antibodies and analyzed by flow cytometry. Percentages of cells in quadrants and total cell numbers are shown within and above plots, respectively. (B) Dot plots show CD44/CD25 subsets in DN-gated cells (top panel), and CD25 expression in total thymocytes (bottom panel). TCR expression in total thymocytes (C) and in thymic and splenic CD4/CD8 subsets (D) of WT (dotted line) and γδ^– × hδTg (solid line) mice. Numbers within histograms indicate mean fluorescence intensity (MFI) ratios of γδ^– × hδTg versus WT cells. (E) Thymic CD4/CD8 subpopulations in CD3γ-deficient and γδ^– × mδTg mice. Numbers within and above plots represent percentages of cells in the gated region and total cell numbers, respectively.