NRP2 overexpression enhances human endothelial cell survival induced by VEGF-A and VEGF-C. HMVECs were transfected with either empty vector (plasmid) or vector containing NRP2. (A) The level of NRP2 expression was assessed by Western blot analysis. (B-C) For survival assay, cells were seeded in triplicate in gelatinized 96-well plates in RPMI containing 0.1% FCS and stimulated with either VEGF-A 10 ng/mL (B) or VEGF-C 100 ng/mL and 300 ng/mL (C). Data are plotted as mean ± SEM. The statistical analysis of differences in VEGF-stimulated responses between empty vector and NRP2-transfected cells was carried out by using ANOVA (***P < .001). Results are representative of 3 independent experiments.