SEMA3F inhibits HMVEC migration induced by VEGF-A and VEGF-C. HMVECs were seeded in triplicate in the upper wells of fluoroblok chambers in the presence of either control or SEMA3F–concentrated media. RPMI alone or RPMI containing either VEGF-A (50 ng/mL) or VEGF-C (100 ng/mL) (A) was added in the bottom wells. Data are shown as mean ± SEM. ***P < .001 versus the respective stimulation of cells incubated with the control media. (B) The bottom wells were filled with RPMI containing 5% FCS. The day after, cells were labeled with calcein, and the fluorescence corresponding to migrating cells was quantified. Results are representative of 2 independent experiments.