Cell-cycle analysis using Ki-67 and 7-AAD. CD34⁺ cells cultured and transduced under survival conditions were sorted based on dGFP⁺ or GFP⁺ expression and assessed for cell-cycle status by Ki-67 and 7-AAD staining. The mock-transduced CD34⁺ cells and sorted mature myeloid CD34^–CD15/33⁺ cells were assessed in the same manner. The percentages of cells within each cell-cycle stage are depicted in density plots. Data presented are from a representative experiment out of 4 independent experiments.