Forskolin blocks augmentation of IL-2 promoter activity by BCL11B. (A) IL-2 mRNA levels in MSCV (□) and MSCV-BCL11B (▪) Jurkat cells stimulated for 4 hours with PMA/ionomycin and pretreated or not with 10 μM forskolin for 30 minutes before stimulation. IL-2 mRNA levels were evaluated by qRT-PCR and normalized by actin in each sample. (B) Ratios of luciferase reporter assays between MSCV-BCL11B and MSCV Jurkat cells transfected with -210 IL-2 promoter (□) or -243 IL-2 promoter (▪). The cells were stimulated with PMA/ionomycin for 8 hours and pretreated or not with 10 μM forskolin for 30 minutes.