BCL11B associates with the p300 coactivator in activated Jurkat cells. Immunoprecipitation (IP) with anti-BCL11B (lanes 3 and 6) or nonspecific (lanes 2 and 5) antibodies and Western blot (IB) analysis with anti-p300 and anti-BCL11B antibodies. Nuclear extracts were prepared from Jurkat cells treated (lanes 4-6) or not (lanes 1-3) with PMA/ionomycin. Lanes 1 and 4 represent input.