Figure 6.
In situ liver perfusion to assess FX involvement in liver hepatocyte transduction. (A) Mouse livers were perfused with PBS to create a blood-free environment, and then 2 × 1011 VP was infused in the presence or absence of human FX at 1 IU/mL. Hepatocytes were then isolated and cultured and reporter gene quantified 48 hours later. (B) Representative images showing β-Gal-expressing cells from the same experiments.
