Effect of warfarin pretreatment on liver transduction after systemic injection and tropism rescue by exogenous FX. (A) Mice were pretreated with 133 μg warfarin 72 and 24 hours prior to injection of AdKO1 virus (4 × 10 VP per mouse) in normal mice versus mice pretreated with warfarin or with warfarin plus 33 μg human FX per mouse and transgene quantified by ELISA. ^*P < .001. (B) X-Gal staining of liver sections; n = 5 animals per group.