Runx1-rescued cells express hematopoietic surface markers. Expression of hematopoietic surface markers of cultured cells at day 12 from Runx1-transduced Notch1-null (Notch1^–/–) embryos (A) or wild-type (Notch1^+/+) embryos (B) was evaluated by flow cytometric analyses. GFP intensity (marking retrovirus-transduced cells) is plotted on the x-axis and intensity of counterstaining of hematopoietic surface markers is plotted on the y-axis. The results show representative results of independent replicates from 5 experiments. Percentages of cells in each quadrant are indicated.