MIP-1 α promoter-driven luciferase was measured in transfected NIH3T3 cells. Luciferase readings were corrected for transfection efficiency by GFP expression and protein concentration. Normalized luciferase activity was expressed as follows: luciferase activity units/(% transfection efficiency × protein concentration). Promoter activity is reduced following treatment with 100 nM PD173074. The experiment is representative of 4 independent experiments with similar results. Error bars are not applied because of large variation in luciferase quantification; however, the range of suppression over 4 independent experiments was 33.3% to 40.5%.