MBD2 occupancy inversely correlates with transcription and histone H3-lysine 4-trimethylation (H3-K4-Me₃) at the ρ- and β^A-globin genes. (A) Enrichment for MBD2, H3-K4-Me₃, and IgG at the ρ-globin gene in 5-day () and adult (▪) erythrocytes as determined by ChIP assay. The data show that MBD2 is depleted from the transcriptionally active ρ-globin gene in 5-day erythrocytes but enriched at the transcriptionally inactive and methylated ρ-globin gene in adult erythrocytes. In contrast, H3-K4-Me₃ is enriched at the transcriptionally active ρ-globin gene and depleted at the transcriptionally inactive gene. No enrichment was seen using anti-rabbit IgG in either 5-day or adult erythrocytes, verifying the interaction of these specific proteins with the ρ-globin gene. The data represent the average of 3 independent experiments, with the SD indicated by the bar. (B) Enrichment for MBD2, H3-K4-Me₃, and IgG at the β^A-globin gene in 5-day () and adult (▪) erythrocytes as determined by ChIP assay. The data show that MBD2 is enriched at the transcriptionally inactive and methylated β^A-globin gene in 5-day erythrocytes but depleted from the transcriptionally active β^A-globin gene in adult erythrocytes. Once again, H3-K4-Me₃ is depleted from the transcriptionally inactive β^A-globin gene and enriched at the transcriptionally active gene. No enrichment was seen using anti-rabbit IgG in either 5-day or adult erythrocytes, verifying the interaction of these specific proteins with the β^A-globin gene. The data represent the average of 3 independent experiments, with the SD indicated by the bar.