EBPs/CLPs fail to generate PDCs on adoptive transfer. (A) Five hundred sorted EBPs/CLPs (Lin^–IL-7Rα⁺AA4⁺Sca-1^lowc-kit^lowFlt3/Flk2⁺) or LSK-Flt3⁺ (Lin^–IL-7Rα^–Sca-1⁺c-kit^high Flt3/Flk2⁺) progenitors from C57BL/6 (Ly5^B6+) mice were transferred intravenously together with 10⁵ unfractionated host-type BM cells into irradiated B6.Ly5^SJL congenics. Host splenocytes were assessed for frequencies of donor-derived (Ly5^B6+Ly5^SJL–) B-lineage (CD19⁺B220⁺) and PDC-lineage (IgM^–CD3^–CD11c⁺B220⁺CD11b^–) cells. Shown are analyses of recipients at day 14 after transfer. (B) Five hundred sorted cells from each population were also transferred via intrathymic injection into B6.Ly5^SJL congenics, and host thymi assessed for donor-derived thymocytes 14 days later. (C) Absolute number of donor-derived splenocytes of the indicated surface phenotype at the indicated time points in mice given 500 EBPs/CLP versus LSK-Flt3⁺ progenitors as described. Data are the mean and SEM using 3 mice per group. Data are representative of 3 separate experiments.