Relative PDC differentiation potential of early myeloid and lymphoid progenitors. (A) c-kit^high ELPs in a 10-week-old NG-BAC transgenic were identified by staining BM cells with the indicated antibodies before collection of 1 000 000 events on an LSRII flow cytometer. (B) A total of 1000 c-kit^high ELPs from NG-BAC transgenics and 1000 CMPs (Lin^–IL-7Rα^–Sca-1^–c-kit^highCD16/32^intCD34⁺; see Figure 5A) from B6.Ly5^SJL congenics were mixed with 250 host-type HSCs (Lin^–IL-7Rα^–Sca-1⁺c-kit^highFlt3/Flk2^–) before transfer into irradiated (C57BL/6 × B6.Ly5^SJL) F1 hosts. Splenocytes from a representative recipient at day 14 after transfer were assessed for the relative contribution of each progenitor population to the PDC lineage by staining cells with the indicated antibodies and collection of 500 000 events. Recipients of host-type HSCs only did not contain detectable Ly5^B6+Ly5^SJL– or Ly5^B6–Ly5^SJL+ cells (not shown). (C) Summary data from 3 recipients per group given either ELPs or EBPs/CLPs mixed with CMPs. Data are the mean and SEM with 5 mice per group and are representative of 2 separate experiments with 5 recipients per group.