Lymphoid gene expression and diminished myeloid potential by a subset of CMPs. (A) BM cells from a 10-week-old NG-BAC transgenic mouse were stained with the indicated antibodies before collection of 500 000 events as described in Figure 1. (B) cDNA was prepared from the indicated populations from NG-BAC transgenics and subjected to PCR with the indicated oligonucleotides. Cell surface phenotypes used for cell sorting were HSCs, Lin^–IL-7Rα^–Sca-1⁺c-kit^highFlt3/Flk2^–; EBPs/CLPs, Lin^–IL-7Rα⁺AA4⁺Sca-1^lowc-kit^low Flt3/Flk2⁺RAG2/GFP^high; pro-B, B220^lowCD43⁺CD19⁺AA4⁺; CMPs, Lin^–IL-7Rα^–Sca-1^–c-kit^highCD16/32^intCD34⁺Flt3/Flk2^+/–RAG2/GFP^+/–; PDCs, CD19^–Ly6C⁺CD11c⁺B220⁺AA4^lowRAG2/GFP⁺. (C) The indicated populations were cultured in methylcellulose medium and characterized as described in “Materials and methods.”