Figure 5.
Generation of PDCs by RAG2/GFPlow CMPs. Five hundred GFP/RAG2+Flt3/Flk2+ or GFP/RAG2–Flt3/Flk2– CMPs from NG-BAC (Ly5B6+) mice were mixed with 2 × 105 host-type BM cells before intravenous transfer into irradiated B6.Ly5SJL congenics. Splenocytes were stained with the indicated antibodies on day 14 after transfer. Data are representative of 2 experiments, both with at least 3 mice per group.