β-Estradiol administration does not perturb PDCs or RAG2/GFP^low CMPs. Analyses of BM progenitor populations in 10-week-old NG-BAC transgenics given transplantations of placebo (cont.) or β-estradiol (E2 Rx) pellets 14 days previously as described in “Materials and methods.” BM populations analyzed included (A) ELPs, (B) EBPs/CLPs, (C) CMPs, and (D) PDCs. Note that in E2-treated mice GFP levels were elevated for both CMPs and PDCs. (E) Absolute number of cells within each indicated gate were calculated by multiplying total cells harvested by the percent viable cells within the indicated gate. Data are representative of 3 experiments. Three mice per group were used to calculate mean and SEM.