Figure 1.
Figure 1. Mutation screening for MPLW515L/K. (A) LightCycler assay for MPLW515L. Melting curve analysis displays that probe bound to a mismatch dissociates at 54°C, whereas probe bound across the wild-type sequence dissociates at 64°C. (B) DNA chromatogram illustrating wild-type MPL515 sequence (i), the TGG→TTG conversion in MPLW515L (ii), the TGG→AAG conversion in MPLW515K (iii), and the presence of all 3 alleles (ie, wild-type MPL, MPLW515L, and MPLW515K) in 2 patients (iv).

Mutation screening for MPLW515L/K. (A) LightCycler assay for MPLW515L. Melting curve analysis displays that probe bound to a mismatch dissociates at 54°C, whereas probe bound across the wild-type sequence dissociates at 64°C. (B) DNA chromatogram illustrating wild-type MPL515 sequence (i), the TGG→TTG conversion in MPLW515L (ii), the TGG→AAG conversion in MPLW515K (iii), and the presence of all 3 alleles (ie, wild-type MPL, MPLW515L, and MPLW515K) in 2 patients (iv).

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