Enhancer activity of –5.5HS. Graphic representation of relative normalized reporter gene activity in cells transfected with constructs as marked. Data from EA.hy926 (▦) and HepG2 (▪) cells are shown. Results for the –5.5HS cloned in both the sense and antisense orientation (–5.5HS/s and –5.5HS/as, respectively) upstream of (A) the SV40 promoter (SV40P), and (B) the hEPCR proximal promoter (PP) are shown. (C) Comparison of the enhancer function of –5.5HS with that of the conserved region within –5.5HS (–5.5HSCR) coupled to SV40P. Data shown are ± SEM (n > 6).