Figure 1.
Bone marrow B cells in C57Bl/6Ly5.1, Rag2°, and Rag2°γc° mice. Bone marrow cells from these mice were stained for CD19 and BP-1. Shown are propidium iodide (PI)–negative cells within the lymphocyte gate. The boxed populations represent 2 subpopulations of the pro–B cells: CD19+BP-1– cells, which correspond to fraction B cells according to the Hardy scheme,17 and CD19+BP-1+ cells, which correspond to fraction C cells according to the Hardy scheme.17 Numbers indicate the percentage of cells in the respective box among lymphocytes.