American Society of Hematology

Figure 1.

$Figure 1. SYK is a substrate of PTPROt in vitro. GST fusion protein binding assays were carried out using GST-PTPROt-WT, -CS, or -DA proteins and pervanadate-treated lymphoma cell lysate. Bound proteins were washed with lysis buffer containing the indicated concentrations of sodium orthovanadate (0-20 mM). Thereafter, the remaining bound proteins were size fractionated, immunoblotted, and probed with the antiphosphotyrosine antibody 4G10 (top panel). The blot was stripped and reprobed with anti-SYK and anti-GST antibodies (middle and bottom panels). Lanes 1 to 3 are total lysates from untreated cells (lane 1, 25 μg protein) or pervanadate-treated cells (lane 2, 25 μg protein; lane 3, 50 μg protein). The last lane is an immunoprecipitation with anti-SYK antibody.$

SYK is a substrate of PTPROt in vitro. GST fusion protein binding assays were carried out using GST-PTPROt-WT, -CS, or -DA proteins and pervanadate-treated lymphoma cell lysate. Bound proteins were washed with lysis buffer containing the indicated concentrations of sodium orthovanadate (0-20 mM). Thereafter, the remaining bound proteins were size fractionated, immunoblotted, and probed with the antiphosphotyrosine antibody 4G10 (top panel). The blot was stripped and reprobed with anti-SYK and anti-GST antibodies (middle and bottom panels). Lanes 1 to 3 are total lysates from untreated cells (lane 1, 25 μg protein) or pervanadate-treated cells (lane 2, 25 μg protein; lane 3, 50 μg protein). The last lane is an immunoprecipitation with anti-SYK antibody.

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