Expression and release of LIGHT from platelets. Flow cytometry analyses of LIGHT detected on resting platelets (gated by anti-CD41) measured as binding of the LIGHT-specific antibody anti-CD258 (A) compared with the binding of an unspecific antibody (B) with the same isotype and concentration. The horizontal line represents the separation of negative and positive events. A representative of 5 experiments is shown. Panel C shows the amount of released LIGHT (pg/108 platelets) measured in the extracellular phase after activation of platelets in PRP by SFLLRN (100 μM). Release from stimulated (•) and unstimulated (○) platelets. Data are presented as mean ± SEM, n = 5. *P < .05 and **P < .01 versus unstimulated platelets at the same time point. Panel D shows amplification plots (real-time PCR) demonstrating gene expression of LIGHT in platelets. Gene expression of β-actin is shown for comparison.
