Figure 6.
Elevated LMO2 expression by activation of the LMO2 proximal promoter. Relative expression of long and total mRNA transcript levels of LMO2 as measured by the RQ-PCR strategy. Long transcripts including the RAG2-LMO2 fusion transcript can be measured by the exon 2/3 primer combination, whereas the total amount of LMO2 transcript was measured using an exon 5/6 primer combination. Expression of proximal promoter transcripts is calculated by subtracting the long-transcript expression from the total expression. FL indicates forward primer long mRNA transcript; RL, reverse primer long mRNA transcript; FT, forward primer total mRNA transcript; and RT, reverse primer total mRNA transcript.