Sensitivity of molecular assay. Sensitivity of (A) AS-PCR, (B) sequencing, and (C) LightTyper melting curves. Genomic DNA isolated from granulocytes of both healthy (G/G) and JAK2 V617F mutated (T/T) patients were mixed at varying ratios respectively: lane 1, 1:1; lane 2, 1:2; lane 3, 1:5; lane 4, 1:10; lane 5, 1:100; and lane 6, 1:500. Lane 7, healthy patient with G/G genotype; and lane 8, CIMF patient with mutated T/T genotype. The 3 methods showed similar sensitivities in detecting a mutated population at a level of 10%.