Figure 2.
Figure 2. Decreased cellularity of E2f4–/– fetal liver is not associated with a block in differentiation or decreased survival. (A) Whole FL cellularity (n = 8 litters) and whole embryo body weight (n = 4 litters) of E15.5 E2f4+/+ and E2f4–/– littermate embryos. Error bars indicate standard deviation from the mean. (B) Immunophenotyping of hematopoietic markers in E2f4+/+ and E2f4–/– E15.5 whole FLs by FACS. (C) Cell size of Ter119+/DRAQ5+ erythroid cells derived from E15.5 FL suspensions was determined by forward scatter measurement by FACS (left plot), and by mean cell volume (femtoliters) parameter on a Sysmex CD4-500 hematological cell counter (Sysmex America, Mundeleine, IL) (right plot); **P < .005, Student t test. Error bars indicate standard deviation from the mean. (D) Measurement of apoptosis by in situ TUNEL assay in E2f4+/+ and E2f4–/– E15.5 FLs. Arrowheads indicate TUNEL-positive cells.

Decreased cellularity of E2f4–/– fetal liver is not associated with a block in differentiation or decreased survival. (A) Whole FL cellularity (n = 8 litters) and whole embryo body weight (n = 4 litters) of E15.5 E2f4+/+ and E2f4–/– littermate embryos. Error bars indicate standard deviation from the mean. (B) Immunophenotyping of hematopoietic markers in E2f4+/+ and E2f4–/– E15.5 whole FLs by FACS. (C) Cell size of Ter119+/DRAQ5+ erythroid cells derived from E15.5 FL suspensions was determined by forward scatter measurement by FACS (left plot), and by mean cell volume (femtoliters) parameter on a Sysmex CD4-500 hematological cell counter (Sysmex America, Mundeleine, IL) (right plot); **P < .005, Student t test. Error bars indicate standard deviation from the mean. (D) Measurement of apoptosis by in situ TUNEL assay in E2f4+/+ and E2f4–/– E15.5 FLs. Arrowheads indicate TUNEL-positive cells.

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