Figure 2.
Figure 2. G-CSF-induced granulocytic differentiation of primary myeloid progenitors. WT and HLF Lin- bone marrow cells were cultured for 7 days in the presence of G-CSF and kit ligand. (A) Representative Wright-Giemsa stains of cytospin preparations from cells on day 7 of culture. (B) Pooled results from 3 independent experiments of manual leukocyte differentials performed on cells on day 7 of culture. Pro indicates promyelocytes; Myle, myelocytes; Meta, meta-myelocytes; Mature, segmented, ring, and band neutrophils. Data represent the mean ± SEM. All images were obtained using a Nikon Eclipse E600 microscope equipped with a Nikon Plan Apo 100×/1.40 numeric aperture oil objective (Nikon USA, Melville, NY). The microscope was equipped with a Sony DXC S500 digital camera (Sony Electronics, Park Ridge, NJ), and images were captured using Kodak Imaging for Windows software (Eastman Software, Billerica, MA).

G-CSF-induced granulocytic differentiation of primary myeloid progenitors. WT and HLF Lin- bone marrow cells were cultured for 7 days in the presence of G-CSF and kit ligand. (A) Representative Wright-Giemsa stains of cytospin preparations from cells on day 7 of culture. (B) Pooled results from 3 independent experiments of manual leukocyte differentials performed on cells on day 7 of culture. Pro indicates promyelocytes; Myle, myelocytes; Meta, meta-myelocytes; Mature, segmented, ring, and band neutrophils. Data represent the mean ± SEM. All images were obtained using a Nikon Eclipse E600 microscope equipped with a Nikon Plan Apo 100×/1.40 numeric aperture oil objective (Nikon USA, Melville, NY). The microscope was equipped with a Sony DXC S500 digital camera (Sony Electronics, Park Ridge, NJ), and images were captured using Kodak Imaging for Windows software (Eastman Software, Billerica, MA).

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