Treatment with poly(I:C) does not affect chemotaxis but increases adhesion to ICAM-1 in vitro. (A) B cells from poly(I:C)– or PBS-treated mice were allowed to migrate toward a gradient of CXCL12 for 3 hours.55 Migrated cells were counted by FACS. Results are expressed as mean ± SD for triplicates per CXCL12 concentration and are representative of 5 experiments. (B) Splenocytes of poly(I:C) or PBS-treated mice were subjected to FACS analysis for the expression of CCR7, CXCR4, CXCR5, CD44, CD62L, CD11a/CD18, CD49d/CD29, and CD69. Histograms are gated on B cells (chemokine receptors) or total lymphocytes (adhesion molecules). Representative data of at least 3 similar experiments are shown. (C) CD43– sorted B cells from naive or poly(I:C)–treated mice were adhered for 30 minutes to plates coated with the indicated concentrations of ICAM-1-Fc or VCAM-1-Fc. Results show mean values of 4 independent experiments (n = 3 each). P values were determined by paired t tests. Horizontal bars represent overall means.
