DCs nucleofected with OVA-encoding plasmids have a highly activated phenotype. At 20 hours after the nucleofection, DCs were collected and analyzed for viability, green fluorescence, and activation markers. (A) Viable (7-AAD^–) DCs nucleofected with GFP-encoding plasmid or without any plasmid were analyzed for green fluorescence. (B) The surface expression levels of CD11c, B7.2 (CD86), MHC class II, and CD40 (gray open histograms) were analyzed from viable cells. Appropriate staining controls are shown as black histograms. (C) DCs nucleofected with pGO become more brightly green fluorescent than DCs expressing pOG.