Nucleofected DCs induce strong antigen-specific immune responses in vivo. (A) Percentage of OVA-reactive T cells was analyzed from naive mice and from mice vaccinated with DCs nucleofeted with pG or pGO. OVA-tetramer-positive CD8 T cells can be found in the spleens of C57BL/6 mice 5 days after the vaccination, only if the DCs used encoded OVA. (B) Spleen cells also exhibit OVA-specific CTL activity when OVA-encoding DCs were used in the vaccination. Results from 3 independent mice per group are shown.