Generation of FOXP3⁺ cells by cytokine matured DCs is contact, CD80/CD86, and IL-2 dependent. (A) T cells were cultured with Cyt-DCs in the presence or absence of transwell inserts. Data (mean ± SD) shown in the left panel are CD4⁺FOXP3⁺ cells expressed as a percentage of control cultures with no transwells. ^*P < .05. The representative FACS plots on the right are from 1 of the 3 experiments summarized in the panel on the left. (B) T cells were cultured with Cyt-DCs treated with anti-CD80 and anti-CD86 antibodies (1-5 μg/mL each), anti-IL-2 antibody (1-5 μg/mL), or isotype control. Data (mean ± SD) shown in the panel on the left are CD4⁺FOXP3⁺ cells expressed as a percentage of control cultures with isotype mAb-treated DCs.^*P < .05. The representative FACS plots on the right are from 1 of the 3 experiments summarized in the panel on the left. The numbers in the FACS plots represent percentage of cells in that quadrant.