PIA assay results compared with combined PKC412, CGP6221, and CGP52421 levels. (A) Standard curve for inhibition of FLT3 autophosphorylation by PKC412 in plasma (Figures 1D and 3B). Superimposed over this graph are PIA assay results (○) plotted against the “adjusted” PKC412 level, calculated by adding the concentration of PKC412 and CGP6221 and value of the CGP52421 level divided by 5.4. (B) The average “adjusted” concentrations of PKC412 for patients on the PKC412 trial. (C) PIA results, averaged for each time point, for patients on the PKC412 trial. Error bars represent standard deviations.