C/EBPα-ER favors monocytic development as assessed by FACS. (A) Marrow cells transduced with pBabePuro or pBabePuro-C/EBPαER, lineage-depleted, and cultured with or without 1 μME2 for 2 days were subjected to FACS for Mac1 and Gr-1. (B) Results from 3 experiments with the indicated vectors. Puro was evaluated in both E2 and 4HT, and C/EBPα-ER was evaluated in several E2 doses. The fold increase in monocytic cells resulting from E2 was obtained as: (% monocytic cells with E2)/(% monocytic cells without E2). For, the C/EBPα-ER data in panel A, this would be (64/64 + 25)/(27/27 + 49). Mean values and SE from 3 determinations are shown. (C) FACS analysis for Mac1 and MPO using C/EBPα-ER–transduced cells. (D) Marrow cells transduced with MIGR1-C/EBPαER, lineage-depleted and then cultured with or without E2 were analyzed for Mac1 and Gr-1 expression within the indicated low-GFP and high-GFP gates on day 3.
